FastQCFastQC Report
Fri 29 Apr 2016
ERR1042831.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042831.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7712050
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT204960.2657659117873976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT195140.25303259185300925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT138870.18006885328803626No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC127880.1658184270070863No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG127560.16540349193794127No Hit
CCCCTATACCTTCCGCATAATGAATTAACTAGAAATAACTTTG111220.14421586996972272No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC104710.1357745346568033No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT103980.13482796403031622No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC101640.1317937513371931No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG98970.1283316368540142No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG97880.12691826427473887No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT90160.1169079557316148No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA83260.1079609183031749No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA78660.10199622668421496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA22500.019.7333326
TAGGGGT22550.019.361424
ATAGGGG23600.018.53
GGGTTAG24500.018.273477
AGGGGTT27300.015.7216125
CTCTAAT36200.015.43370151
TCTAATA38500.015.1844152
GGTAAAC34900.015.16045935
TACTGGT37850.015.1519167
AATACTG38750.015.1341935
GGTATCA118450.014.9468141
CTTGCTA27150.014.92265116
AACTAGA27000.014.66296327
GGTTAGT32250.014.6279078
ATGCTAG38350.014.61668815
TAACTAG27550.014.50453726
TAATACT42750.014.4970764
ATACTGG40200.014.4502486
TTAACGG31650.014.26224335
ATTAACT28450.014.24077424
Produced by FastQC (version 0.11.5)