FastQCFastQC Report
Fri 29 Apr 2016
ERR1042830.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042830.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7211965
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT195780.2714655437179742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT180810.25070837143552416No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT123160.17077176608594188No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG72260.10019460715630206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA11000.015.6409096
TAGGGGT11200.015.0312494
GGGTTAG12200.014.2540997
GTATAGG17600.013.9801141
ATAGGGG12450.013.8192763
TAGACTA4300.013.7674425
TAATACT17550.013.4928784
TTAACGG11400.013.46929835
GGTTAGT14900.012.2919468
TATACTG7950.012.1006295
TTAATAC6600.012.0530313
TAACGGC12600.012.03968236
TATTAGA18050.011.991692
GTTAGTC12850.011.8054489
GTATTAG22900.011.7139741
ATTAGAG15500.011.69677453
GTATTAA7550.011.5165571
CGAACTA12150.011.41975329
TTAGACT5850.011.3846164
CCTCTAG17500.011.3114291
Produced by FastQC (version 0.11.5)