FastQCFastQC Report
Fri 29 Apr 2016
ERR1042829.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042829.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6277714
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121270.193175413852877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115860.18455762718722132No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77730.12381895702798822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA11900.015.3907576
TAGGGGT11600.014.8318974
TTAACGG13700.013.63868635
GTATTAG23650.013.5327691
GGGTTAG13550.013.38007457
TATTAGA17200.013.3372112
TAACGGC14200.012.89788736
ATAGGGG13200.012.8939393
ATTAGAG16000.012.6031253
TAATACT16750.012.5910444
TAACTAG12500.012.43226
GGTATCA94000.012.4186171
AACTAGA12450.012.33333327
TCTAATA15600.012.0961542
TAGACTG6950.011.9784175
AGGGGTT14500.011.7379315
GTATAGG20400.011.698531
GTTTAGG12500.011.6921
GGTTAGT16750.011.5970148
CTTACAC9950.011.5276383
Produced by FastQC (version 0.11.5)