FastQCFastQC Report
Fri 29 Apr 2016
ERR1042828.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042828.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7367088
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT362330.49182254915374973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT319410.433563437819665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238920.3243072432418345No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98830.1341506983491985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG15500.016.35161235
TAACGGC16200.015.53086436
GGGGTTA15900.014.8930836
GGTATCA152250.014.8243021
GGGTTAG16150.014.6625397
ATAGGGG16450.014.50763
TAGGGGT17000.014.0382364
TATTAGA23650.013.6892182
GTATTAG30950.013.6284321
AACTAGA18700.013.55347627
ATTAGAG21750.013.3540233
TACCCCG10850.013.1290325
CTAGTAC3800.012.6578953
GGGTAAG11150.012.6098651
TAGGACC16300.012.5981594
TTAGGAC15600.012.5705133
TTAACTA19750.012.45822825
AACGGCC20350.012.45454537
ATGCTAG20250.012.42469115
TAATACT26650.012.2176354
Produced by FastQC (version 0.11.5)