FastQCFastQC Report
Fri 29 Apr 2016
ERR1042827.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042827.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6615668
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79650.12039600536181683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79250.11979138009948503No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55150.015.9338171
TTAACGG7900.013.34810135
TAGACCG1409.574436E-613.2142855
TATACTG7550.012.4966895
TAACGGC8500.012.18823436
CTTACAC8200.011.9573163
GTATCAA73500.011.8299322
TAATACT10800.011.8194454
ATTAGAG11500.011.5826083
GTATTAG18000.011.5111111
CTAGTAC3900.011.3846163
GTATAGA5850.011.3846161
GGGGTTA7400.011.256
TATAGTG6500.011.0999995
TAGGGGT7450.010.9261744
GTATAGG9850.010.8934011
TACACCG6800.010.8823535
GGGTTAG8000.010.8687517
CTACACT13200.010.7916674
TATTAGA12200.010.7663942
Produced by FastQC (version 0.11.5)