FastQCFastQC Report
Fri 29 Apr 2016
ERR1042826.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042826.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10933227
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT427320.3908452646231529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT380480.34800338454511187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT222320.2033434410535883No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183060.16743455523241216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG13850.014.02527135
TATACTG14800.013.8750015
GTACTAG7000.013.4785711
TAATACT24400.013.3442624
GTATTAG29200.013.1147261
TAGGGGT13200.012.6136364
CTAGTAC7900.012.4113923
TCTATAC9950.012.2713573
TAACGGC15850.012.13880136
ATAGGGG14600.012.0376713
CTAATAC30900.012.033983
TATTAGA23300.011.9098722
GGGGTTA13500.011.7851856
ATTAGAG20350.011.7272733
GTATAGG22800.011.6842111
TCTAATA21600.011.56252
AATACTG28750.011.4539135
CTCTAAT21250.011.2305881
TCTAGAC9900.011.2121213
TCTAACG3301.0913936E-1111.2121212
Produced by FastQC (version 0.11.5)