FastQCFastQC Report
Fri 29 Apr 2016
ERR1042825.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042825.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8634869
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT289130.33484005374024783No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT269500.31210664574065916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT155700.18031541648170923No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121330.14051168581712126No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG107670.12469210592540549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT16750.015.3522394
GGGGTTA16950.015.0619486
ATAGGGG16800.014.5357143
GTATTAG28850.014.17157651
GGGTTAG17450.014.10028657
TAATACT25900.014.0714274
CTCTAAT23300.014.0536481
TCTAATA24200.013.6838852
ATTAGAG21900.013.5159833
TTAACGG17700.013.4830535
AATACTG29400.013.4659875
TATTAGA23750.013.322
CTAGTAC5500.013.1181813
TAACGGC18200.013.11263736
ACGCGTT850.009411219513.05882331
CTAATAC32050.012.8143533
GGTAAAC22650.012.74172135
GGGTAAG10300.012.5728161
ATGCTAG23800.012.35924415
CCCCTAT19300.012.1735741
Produced by FastQC (version 0.11.5)