FastQCFastQC Report
Fri 29 Apr 2016
ERR1042824.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042824.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8365303
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT449930.5378526037849436No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT423700.5064968955697122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT254740.30451975260190817No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181470.21693177162859495No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG87110.10413251020315702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG13050.015.31034635
TAGGGGT12150.015.0740744
GGGGTTA13450.014.9925656
CTACACT16350.014.3700314
TAACGGC13850.014.29241936
GTACTAG4950.013.8282831
ATAGGGG14000.013.4785713
GGGTTAG14550.013.4776637
GTATTAG26500.013.2641511
TATACTG11750.013.0680865
TAATACT26050.013.0671784
TAACTAG18200.012.40109826
CTATACC23150.012.3866084
TCTAATA24500.012.3081642
CCTATAC26250.012.2628573
GTATAGG25500.012.2607851
GGTAAAC21250.012.18823535
TTAGGAC14450.012.1626293
CCCCTAT20850.012.1558751
TATTAGA21950.012.0523912
Produced by FastQC (version 0.11.5)