FastQCFastQC Report
Fri 29 Apr 2016
ERR1042823.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042823.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9030306
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT340310.37685323177309826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT328730.36402974605733185No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT211830.23457676849488823No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133400.14772478363413155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT19450.018.262214
GGGGTTA18500.017.96
GGGTTAG20000.016.8357
TTAACGG18800.015.84308535
ATAGGGG21450.015.3519823
TAACGGC19200.015.22395836
TAATACT32250.014.5131794
GTATTAG33950.014.1134011
CTCTAAT26500.013.8226421
TCTAATA30600.013.7843132
AATACTG33050.013.6580945
GGGTAAG12450.013.5220881
ATTAGAG25150.013.4612323
ATACGAC2202.8558134E-1013.4545453
GGTTAGT26050.013.28023058
AGGGGTT25750.013.0038835
CTTGCTA24050.012.92307716
TTTAACG24300.012.79012334
ATGCTAG27500.012.78181815
CTAATAC39250.012.6789813
Produced by FastQC (version 0.11.5)