FastQCFastQC Report
Fri 29 Apr 2016
ERR1042822.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042822.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10145095
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT371480.3661670984845386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT361410.3562411194769492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT239960.23652809559693627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145400.1433204913310324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA22650.016.0905086
TAGGGGT23050.015.0086774
GGGTTAG24000.014.7229167
ATAGGGG24450.014.3762783
TTAACGG24400.014.32991735
TAACGGC24600.014.21341436
GTATTAG43250.014.1583811
TATTAGA32600.013.73312952
TAATACT36050.013.2912634
ATTAGAG30850.013.0129673
GGTATCA215300.012.9405481
AATACTG36150.012.845095
AACTAGA29400.012.71088527
CTCTAAT30650.012.6753661
ATGCTAG29700.012.58249215
TTAACTA29700.012.58249225
TCTAATA32550.012.5606762
ATTAACT29600.012.37500124
GGTTAGT29450.012.3123938
TACTGGT33300.012.1666677
Produced by FastQC (version 0.11.5)