FastQCFastQC Report
Fri 29 Apr 2016
ERR1042821.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042821.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10049022
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT256750.25549750015474143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT224060.2229669713132283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT162500.16170727857895026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105050.016.5188031
TAATACT21000.013.4785724
TTAACGG9750.013.47179535
TATACTG15500.013.3677435
GTATCAA140950.012.33770752
CTACACT20450.012.0317854
GTATTAG24450.012.0306751
TAACGGC10950.011.99543436
GGGTAAG10100.011.9059411
TCTAATA18550.011.4690032
ATAGGGG11550.011.3722943
AATACTG27250.011.3376155
GTCGTAC4900.011.3265311
TAGGGGT12000.011.2541674
GTGTAAG16600.011.1445781
TATACCG5050.010.9900995
CTAATAC27100.010.8542443
TACACTG27800.010.8471225
CTCTAAT18250.010.8465751
TCTATAC11150.010.7847543
Produced by FastQC (version 0.11.5)