FastQCFastQC Report
Fri 29 Apr 2016
ERR1042820.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042820.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8283654
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135070.16305606197458272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121590.14678305008876516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87340.10543656217413234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64200.018.9898761
GGGGTTA13750.015.6072726
TAGGGGT14900.015.27181154
ATAGGGG14250.014.9298253
CTTACAC11600.014.8318973
GGGTTAG14650.014.7747457
GTATAGG20400.014.1470591
GTATCAA89150.013.592262
TTAACGG12150.013.55144135
TATTAGA17650.013.3116152
TATAGGG19850.013.0478592
GTATTAG23550.012.7261141
TAACGGC13050.012.61685836
GGTTAGT17500.012.3685718
TAATACT21150.012.1583924
CCTATAC20200.011.9059413
CTCTAAT18350.011.8964581
GTTAGTC16350.011.7675859
CTACACT16900.011.7130174
GTTTAGG14550.011.6975951
Produced by FastQC (version 0.11.5)