FastQCFastQC Report
Fri 29 Apr 2016
ERR1042819.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042819.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10283611
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT509230.4951859808777287No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT445270.4329899293156849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT270940.2634677643874316No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192920.18759947259770912No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG109460.10644121019357888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA26550.017.2109226
TAGGGGT27550.016.7205094
TAGACCG1251.6613558E-716.2799995
GGGTTAG28950.016.2314347
ATAGGGG29350.014.9386713
TAATACT35750.013.7650364
TATTAGA30600.013.6633992
ATTAGAG28600.013.5839163
CTTGCTA30000.013.56666816
AATACTG35700.013.473395
TCTAATA32050.013.3338542
GGTTAGT35400.013.3262728
GTATTAG41000.013.2658551
CTCTAAT31600.013.1139251
CTAGTAC6350.013.1102363
AGGGGTT34350.013.0334795
GGTAAAC27500.012.91636435
AACTAGA28000.012.88392827
GTATAGG46850.012.8335111
TAACGGC23750.012.77473736
Produced by FastQC (version 0.11.5)