FastQCFastQC Report
Fri 29 Apr 2016
ERR1042818.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042818.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11372415
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT280470.2466230787392124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT265750.23367947792970975No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181070.15921860044678285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA26900.017.193316
ATAGGGG27050.016.4140473
GGGTTAG28400.016.154937
TAGGGGT28700.016.1149834
GGTATCA140200.015.3726821
TAACTAG30650.014.30505726
TTAACGG27550.014.23593435
ATTAGAG32650.013.9954073
TAATACT42000.013.8309534
TATTAGA34550.013.7612162
TCTAATA38550.013.7250332
GTATTAG43500.013.6091961
GGGTAAG14750.013.420341
AGGGGTT34050.013.3113075
CTCTAAT34750.013.3093531
AACTAGA33950.013.29602427
TAACGGC29550.013.20981336
CTTGCTA31450.013.11764716
GGTAAAC33050.013.0423635
TTAACTA34750.012.93669125
Produced by FastQC (version 0.11.5)