FastQCFastQC Report
Fri 29 Apr 2016
ERR1042816.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042816.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13274315
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT341380.25717334566793093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT322370.2428524560401045No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT223400.1682949364995482No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG219240.16516106480824058No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT161340.1215429948739351No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141970.10695090481128405No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG139010.1047210345693921No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG132850.10008049379572506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG12600.013.65476135
CTACACT27600.013.0706524
GTATTAG26400.012.8238641
TAACGGC13850.012.28880936
TATACCG6600.012.053035
TAGACTG12200.011.8278685
CTAGTAC6900.011.7971013
TATACTG19150.011.3028725
TAATACT27050.011.0794834
TACACTG36100.011.0692525
CTAATAC27700.011.01985553
TAGGGGT16450.010.7963534
TTTAACG18000.010.68888934
GTACTAG7100.010.6830991
GTATAGG22100.010.6312221
GGGGTTA15200.010.5888166
TATTAGA24750.010.5393932
TCTAGAC15100.010.5364253
GTCTAGA13550.010.3763841
TCTATAC14500.010.3344823
Produced by FastQC (version 0.11.5)