FastQCFastQC Report
Fri 29 Apr 2016
ERR1042815.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042815.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5267754
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT207290.3935073657577784No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT201040.381642726672506No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139690.26517942941147216No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96410.18301917667377787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102800.014.4148831
TAACGGC6750.013.977777536
TTAACGG6800.013.602941535
TAGGGGT8050.013.3291934
GTATTAG13250.012.42641451
GGGGTTA7000.012.4214286
TAATACT14850.012.2087544
CTAATAC13900.012.111513
CGGACTA2654.2564352E-1011.8679259
TTTAACG8800.011.35227334
TATACTG7000.011.0999995
ATTAGAG10550.011.0473933
CGTCTAA1350.001376391610.9629631
GGGTAAG6500.010.8153851
GTATAGG12050.010.7468881
CTCTAAT11600.010.6853451
ATAGGAC4850.010.6804123
TATTAGA12300.010.6788622
GCTATAC3301.3096724E-1010.6515153
GTATCAA139050.010.6436532
Produced by FastQC (version 0.11.5)