FastQCFastQC Report
Fri 29 Apr 2016
ERR1042814.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042814.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17490752
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT631150.36084783547328325No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT616370.3523976556296722No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT424360.24261964265458683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT294600.16843186616561712No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG216580.1238254364363522No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC199590.1141117317311457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT40250.015.8111794
GGGGTTA39200.015.6683666
TTAACGG50200.015.22011935
GGGTTAG41600.015.1646627
TAACTAG47000.014.99680826
TAACGGC51900.014.6502936
ATAGGGG41150.014.6111783
GGGTAAG26500.014.3811321
AACTAGA50200.014.33565727
GTATTAG80200.014.0941391
ATTAGAG63550.014.0605813
TATTAGA69400.014.0482712
CTCTAAT65650.013.8644321
GGTAAAC61150.013.79558535
TTAACTA52450.013.75595825
ATTAACT51600.013.659883524
TAATACT79500.013.6364784
TCTAATA73650.013.5892732
ATGCTAG64750.013.34285715
TTTAACG59700.013.29396934
Produced by FastQC (version 0.11.5)