FastQCFastQC Report
Fri 29 Apr 2016
ERR1042813.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042813.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13413533
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT289240.21563297305788118No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT260560.1942515815930076No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT200020.14911805860543975No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG151460.11291581420047947No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC141510.10549793257302159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA29300.018.0580226
TAGGGGT31650.016.8341244
ATAGGGG31600.016.4509493
GGGTTAG32650.016.4318527
TAACTAG35650.015.61991726
TTAACGG35800.015.60614435
TAACGGC36700.015.07220736
GTATTAG56150.014.92521
GGTATCA158350.014.41681
CTCTAAT46150.014.3510291
ATTAGAG44300.014.1151253
TAATACT54550.014.0742444
TATTAGA47600.013.9527322
CCCCTAT43900.013.9066061
GTATAGG51450.013.8794951
TCTAATA50450.013.8245782
TTAACTA40600.013.8066525
AACTAGA40350.013.75464727
AGGGGTT39400.013.6167525
TTTAACG42400.013.48231134
Produced by FastQC (version 0.11.5)