FastQCFastQC Report
Fri 29 Apr 2016
ERR1042811.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042811.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15015879
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT391540.2607506360433512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT366210.2438818266982572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT251860.16772910863226856No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC194600.12959614285650542No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG190010.12653937874699178No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167980.11186824294468542No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG163970.10919773660935866No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG151890.10115291951939677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT35750.016.1454544
GGGGTTA33250.016.024066
GGGTTAG34050.015.6475777
TTAACGG51500.015.08737835
ATAGGGG37550.014.9773653
GGTATCA201300.014.7411831
TAACGGC52950.014.63928336
ATTAGAG58600.014.5221843
TATTAGA64250.014.4832692
GTATTAG72950.014.4551061
GGGTAAG24150.014.1718431
TTTAACG58150.013.5528834
TAACGCC3750.013.324
CTCTAAT55600.013.2760791
GGTAAAC52400.013.13358835
CGAGACG14400.013.10416723
AACGGCC60300.013.03897337
TCTAATA61700.012.9529992
CTAATAC71100.012.8016873
GTAAGAT26750.012.7252343
Produced by FastQC (version 0.11.5)