FastQCFastQC Report
Fri 29 Apr 2016
ERR1042810.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042810.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15074829
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT993190.6588399775546376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT787770.522573091873878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT497890.3302790366643628No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411120.27271951144520445No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG233460.15486742834694842No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT153970.1021371453036051No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG153170.10160645935021882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG50200.014.8147411
GGGGTTA28000.014.4696436
TAGGGGT29900.014.2926424
TTAACGG33950.014.0589135
TAACGGC34350.013.78748136
TAATACT53550.013.4388424
ATTAGAG43200.013.2754623
TATTAGA47550.013.2670872
ATAGGGG30700.013.0765473
CTCTAAT45200.013.0564151
TAGACCG2850.012.9824565
AATACTG57600.012.879345
GGGTTAG31900.012.8746077
TCTAATA50100.012.8502992
CTAATAC54250.012.7539183
TTTAACG40600.012.43965534
ATGCTAG43050.012.4192815
TACTGGT50500.012.4188127
GGGTAAG22650.012.4150111
ACCGCCT44750.011.90614512
Produced by FastQC (version 0.11.5)