FastQCFastQC Report
Fri 29 Apr 2016
ERR1042808.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042808.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17998178
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT620340.3446682214166345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT613460.3408456122614189No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT408500.22696741859092626No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264860.14715934023988428No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG221250.12292910982433888No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC203510.11307255656655914No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG195120.10841097359966104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG53850.015.97493135
TAACGGC54550.015.6342836
GGGGTTA38250.015.283666
TAGGGGT39300.015.0636144
GTATTAG81100.014.7817511
CTCTAAT69900.014.6888421
ATTAGAG68450.014.4324323
TCTAATA75800.014.1556732
ATAGGGG41600.014.1418273
TATTAGA73800.014.1382112
TTTAACG62250.014.08674734
TAATACT85200.014.0487094
CTAATAC86950.013.8510643
GGGTTAG41950.013.8474377
AACGGCC62000.013.81532337
ATGCTAG70500.013.6453915
TAACTAG52250.013.52535826
TACTGGT77700.013.4047627
GATGCTA72400.013.23618814
AATACTG87550.013.1433485
Produced by FastQC (version 0.11.5)