FastQCFastQC Report
Fri 29 Apr 2016
ERR1042807.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042807.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9353936
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG303600.32456925084798527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT298260.3188604241038211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT294240.31456276801551775No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT228090.2438438749206751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT207690.22203487387555354No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG194530.2079659300640928No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG186290.1991568041517496No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG168330.17995633068261319No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT148200.1584359781807359No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT143580.15349688088522306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137840.14736042666958593No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG134900.14421736475425959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT13150.014.9125484
TTAACGG11950.014.70711235
GGGGTTA13650.013.8241766
TAATACT26050.013.7063354
TAACGGC12550.013.56175236
ATAGGGG13500.013.4296293
GTATTAG24750.012.7818171
GGGTTAG14000.012.5535727
CTCTAAT21550.012.3619481
GTATACG3051.8189894E-1212.1311471
TTAGGAC13600.011.83455853
CCCCTAT17050.011.826981
AATACTG31250.011.78085
TCTAATA23900.011.6882842
CTAATAC28800.011.6267373
CGAACTA9550.011.62303629
GTATAGG23000.011.5021741
TTTAACG17000.011.42647134
TAGGACC15400.011.2922084
ATTAGAG18950.011.22691253
Produced by FastQC (version 0.11.5)