FastQCFastQC Report
Fri 29 Apr 2016
ERR1042806.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042806.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9356463
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT269720.28827132646171955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT225960.2415015161177894No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT185710.19848312337685725No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111620.11929721733522593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC10200.012.87745136
GTATTAG24100.012.8195031
TTAACGG10900.012.05045935
GGGTAAG8950.011.9888271
TAATACT23650.011.5771674
TAGGGGT12150.011.5720164
GTATAGG20000.011.56251
CTATACC20000.011.4699994
CTACGCT2757.876224E-1011.4363634
CCCCTAT15750.011.3936511
CTAATAC25350.011.2386593
TCTAATA21050.010.8978622
CCTATAC22700.010.8392073
CTACACT17000.010.773534
TCTATAC10500.010.7476193
TACACTG25000.010.6565
TAACTAG15350.010.48534226
TACGGGT20900.010.4449764
CTAGACA11700.010.4358984
TAGTACT11000.010.4272724
Produced by FastQC (version 0.11.5)