FastQCFastQC Report
Fri 29 Apr 2016
ERR1042805.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042805.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7321909
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT326450.445853670128924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT311150.42495748035109426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT229040.3128145952100743No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG227070.310124040055674No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT172190.23517090966304008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159570.2179349675064249No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG136130.18592145846117453No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG126830.17321985290994465No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG122320.16706025709961705No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT96040.1311679781871094No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT91650.1251722740613138No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG88470.12082914442121584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC13100.015.5343536
TAGGGGT12050.015.5062244
TTAACGG13250.015.49811435
TAACGCG750.00410720714.79999923
GTATTAG25750.014.4407771
GGGGTTA13200.013.8756
TATCCCG2151.9826984E-1013.7674425
GGGTTAG13550.013.6531377
GGGTAAG10800.013.5324081
TAATACT30000.013.324
CTCTAAT25050.013.2934141
AACGGCC15350.013.25732937
ATAGGGG14200.013.158453
TTTAACG16650.013.11111334
GGTAAAC20900.013.10047835
TCTAATA27650.012.91322
CTAATAC31050.012.6312393
TAACTAG17050.012.58650926
ATGCTAG23050.012.36008715
CCGAGTT9750.012.33333313
Produced by FastQC (version 0.11.5)