FastQCFastQC Report
Fri 29 Apr 2016
ERR1042804.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042804.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6705240
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT294450.43913416969414965No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT288940.4309167158819073No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT225430.3361997482565874No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137110.20448186791225967No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG120410.1795759734178046No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT85670.12776574738562677No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG74520.11113696154052652No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG72120.10755767131377847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG9700.014.30412435
GTATTAG18400.013.9755441
TAATACT22100.013.8959264
TAACGGC10000.013.87536
TAACTAG10850.013.8110626
TAGGGGT8600.013.5523274
CTATACC16100.012.9844724
TATACCG2850.012.9824565
CCTATAC16400.012.746953
GGGGTTA9100.012.4010996
TACTCCG12450.012.3333335
CTAATAC20850.012.3333333
CCCCTAT14050.012.1138791
TCTATAC9200.012.0652183
GGGTTAG8500.011.9705887
TAGGACA7000.011.8928574
CATACGA1257.226703E-411.8414
TCTAATA19050.011.7506562
GGTCGCC14200.011.72535236
GTATAGG14850.011.7104381
Produced by FastQC (version 0.11.5)