FastQCFastQC Report
Fri 29 Apr 2016
ERR1042803.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042803.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11111825
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT306390.2757332841364942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256870.23116814744652656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT219770.19778029261619942No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122880.11058489492050136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG20700.015.81884135
TAGGGGT19050.015.635174
TAACGGC21650.015.03926136
TAATACT37550.014.2876164
ATAGGGG19250.013.9350653
GGGGTTA18650.013.5898126
GTATTAG40550.013.5499381
GGGTTAG20350.013.3636367
TCTAATA36150.013.15214352
CTCTAAT33050.012.93040851
ATTAGAG31150.012.8876413
TTTAACG27700.012.75631734
AACGGCC26450.012.72967837
GGTAAAC30850.012.59319235
ATGCTAG31950.012.33333415
AATACTG41600.012.318515
GGGTAAG16100.012.2950321
GTATAGG32200.012.2375771
CTAATAC47350.012.1510033
TATTAGA36900.012.0826562
Produced by FastQC (version 0.11.5)