FastQCFastQC Report
Fri 29 Apr 2016
ERR1042801.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042801.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6656433
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365690.5493783231950206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT354530.5326125869516No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT253100.3802336777069641No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179240.2692733480529287No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG67620.101585939496424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG28650.014.7870861
TTAACGG17300.014.75722535
TAACGGC17750.014.38309836
GTACCGT1956.7484507E-1014.2307686
TATTAGA25000.013.9122
TAATACT29400.013.8435374
GGGTAAG8700.013.8218391
AACGGCC19300.013.41968837
CTCTAAT23050.013.242951
ATTAGAG21250.013.1458833
TCTAATA25650.013.0545822
AATACTG28800.013.039935
GGTAAAC21800.012.98394635
CTAATAC30450.012.8801323
GATGCTA23550.012.64755814
ATACTGG26050.012.4280226
TACTGGT26100.012.4042157
TTTAACG21000.012.33333334
TACGGGT15000.012.3333334
TAGGGGT11550.012.3333334
Produced by FastQC (version 0.11.5)