FastQCFastQC Report
Fri 29 Apr 2016
ERR1042800.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042800.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12547277
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT320110.25512308367783704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT319800.2548760181193099No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT210790.16799660994174273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132560.10564842076890468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG33500.014.91044935
TAACGGC34650.014.2554136
ATTAGAG41150.013.5771573
GTATTAG55100.013.5644281
CTCTAAT39350.013.49301051
TAATACT48500.013.4649494
GGTAAAC36250.013.11586235
TAGGACC25300.013.0158114
TTAGGAC27250.012.8990823
AATACTG49650.012.8922465
TCTAATA45400.012.5506612
CTAATAC56100.012.5311943
AACGGCC40050.012.42571837
TATTAGA47750.012.3204192
ATGCTAG40400.012.31806915
GGGTAAG18950.012.3007921
ATACTGG48150.012.17964656
TACTGGT46950.012.1757197
TTGGTAA40500.012.15061833
TTTAACG41900.012.09785234
Produced by FastQC (version 0.11.5)