FastQCFastQC Report
Fri 29 Apr 2016
ERR1042799.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042799.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13115272
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT634290.48362702656872075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT600690.45800803826256903No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT401290.3059715421837992No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247520.18872654718865153No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG228570.17427774277193792No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC204450.15588696902359325No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC180410.1375571928664537No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG172860.1318005451964702No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC166420.12689023910445776No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG162830.12415297219912785No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT155240.11836582573354179No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA136100.10377215203771603No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT132670.10115688031479637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT56850.015.9780121
ATTAGAG58800.015.9200693
TTAACGG54750.015.91506935
GGTATCA264250.015.8571421
TAACGGC55100.015.74682436
TATTAGA69100.015.2337192
GTATTAG79300.015.2105931
GTGACAC60400.014.94702124
AACGGCC58750.014.8314937
AATACTG69200.014.7304915
TTTAACG60700.014.59884734
TCTAATA72600.014.4228662
TAGGACC32000.014.3953134
GACACAT62500.014.35626
GGTAAAC61500.014.25853635
CACATGT63550.014.17702728
ATACTGG69050.014.1462716
GAGGCAC65800.014.0858657
GGCACCG63600.013.9913529
CTAATAC81050.013.7180763
Produced by FastQC (version 0.11.5)