FastQCFastQC Report
Fri 29 Apr 2016
ERR1042798.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042798.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13059081
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT505190.3868495799972448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT421130.322480578840119No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT309860.23727550200507985No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172030.13173208742636638No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG148370.11361442662006616No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC135730.1039353381757874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA201250.016.7304341
TTAACGG34400.015.16569735
TAACGGC35750.014.54125936
CTCTAAT48100.014.01
GTATTAG58850.013.6745971
ATTAGAG45800.013.5316593
GGTAAAC46950.013.2790235
AATACTG58700.013.2052815
AACGGCC39900.013.07518837
CTAATAC64700.012.9528593
TAATACT63500.012.9062994
TATTAGA51350.012.789682
TCTAATA57000.012.6578942
ATGCTAG49750.012.64321615
TTTAACG43200.012.59027834
GGGGTTA26400.012.4034096
ATAGGGG25750.012.3572813
GATGCTA52050.012.29779114
CCGAGTT16250.012.29538413
TACTGGT55450.012.2777287
Produced by FastQC (version 0.11.5)