FastQCFastQC Report
Fri 29 Apr 2016
ERR1042797.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042797.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15400497
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1051630.682854585796809No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT925710.6010909907647786No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT590440.38339022435444775No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT436290.28329605206896896No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG281300.18265644284077326No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC253120.1643583320720104No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC216620.1406577982515759No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG199760.1297100996156163No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG192390.12492453977296966No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC192040.12469727438017097No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT177340.11515212788262613No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA159490.1035615928498931No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAT155650.10106816682604464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG62000.016.73951535
TAACGGC62200.016.59646436
ATTAGAG69800.016.2471333
CCGAGTT20650.015.85714313
CTCTAAT72950.015.7230991
AACGGCC66300.015.6817537
TATTAGA79850.015.4301822
CGAGTTC21400.015.21495414
GGGGTTA44750.015.0893866
GGTAAAC72100.015.087378535
GTATTAG96950.014.9412061
GTGACAC70750.014.87844524
AATACTG82800.014.8580925
GACACAT71650.014.84647726
TTTAACG72400.014.66712734
GCCGAGT22250.014.63370812
GTAAACA76050.014.547008536
CACATGT72800.014.45947928
TGACACA72850.014.44955425
GTTTAAC72950.014.42974833
Produced by FastQC (version 0.11.5)