FastQCFastQC Report
Fri 29 Apr 2016
ERR1042796.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042796.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12211440
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT339840.2782964171301665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT303140.24824263149964293No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT206380.16900545717785945No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG139070.11388501274214999No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC132610.10859489134778534No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125190.10251862188243156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA163950.015.3461431
TTAACGG33900.015.17109235
CTCTAAT40000.015.171
TAACGGC34400.014.78924436
ATTAGAG41750.014.0467063
GTATTAG57900.013.9948191
AATACTG52050.013.96835
TAATACT54300.013.8664834
TCTAATA47450.013.723922
TATTAGA46200.013.6147182
TTTAACG40200.013.575870534
TACCCCG20250.013.1555565
GGTAAAC44300.013.15462835
GGGTAAG17350.013.1152731
AACGGCC39750.013.03144637
ATACTGG52250.013.0296666
TACTGGT49300.013.0212987
CTAATAC57150.013.0131233
ATGCTAG45150.012.9479515
GGGGTTA25150.012.6520876
Produced by FastQC (version 0.11.5)