FastQCFastQC Report
Fri 29 Apr 2016
ERR1042795.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042795.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9122260
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365810.40100808352316203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT357300.39167925492147776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237100.25991366174610236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150370.16483853781847918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG33900.014.0250741
TTAACGG17950.013.70752135
TAACGGC18400.013.37228336
CTCTAAT22500.013.2377771
GTACTAG5600.012.8839281
ATTAGAG23400.012.6495733
TAATACT31350.012.6283894
TATACTG13550.012.4243545
CTAATAC33550.012.4068553
GTTTAGG22800.012.3333341
TAGGACC18750.012.2346664
TATTAGA28450.012.0949032
TCTAATA28300.012.0282692
GGTATCA212950.011.9800431
TTAGGAC17900.011.8854753
ATTTCGG16400.011.84451229
TTAGAGG34700.011.8357354
CTAGTAC6100.011.8278693
GTATACG4400.011.7727271
GGGGTTA17800.011.7443826
Produced by FastQC (version 0.11.5)