FastQCFastQC Report
Fri 29 Apr 2016
ERR1042794.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042794.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13176025
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT431060.3271548133826401No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT408390.3099493208308272No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT256260.19448961276257443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166760.12656320855493217No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG20000.013.41249935
GGTATCA238950.013.2778821
TAACGGC20900.012.83492836
GTATTAG39050.012.6491671
TATACTG19900.012.3643225
TAATACT42350.012.2750874
GTATAGG31850.011.7331241
CTAATAC43450.011.708863
GGGTAAG17600.011.6676141
TAGGACC23150.011.507564
TCTATAC14800.011.53
GTCGTAC6000.011.4083341
TAGGGGT21250.011.3176464
CCTCTAG33050.011.3071111
CTACACT25900.011.2142864
AATACTG42500.011.18705755
TTTAACG25500.011.17254934
TATTAGA36500.011.0999992
TCTAATA36500.010.998632
ATTAGAG29450.010.9932093
Produced by FastQC (version 0.11.5)