FastQCFastQC Report
Fri 29 Apr 2016
ERR1042793.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042793.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6315331
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT277430.4392960558995245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT245290.38840402822908254No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165880.26266240043475153No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101870.161305876129058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107000.016.7191581
GTACCGT1854.9112714E-914.06
TTAACGG10850.013.8110635
TAACGGC11200.013.70982136
GTATTAG18150.013.6584031
TAACGCC2204.096364E-912.6136364
TCTAACG2204.096364E-912.6136362
CTAGTAC4350.012.3333343
ATTAGAG14850.012.3333333
GTATCAA145700.012.2656142
TATTAGA18250.011.9616432
TACACTG16600.011.9246995
TAGACCG1257.2265975E-411.845
CTAATAC20800.011.8293273
GTACTAG3550.011.4647891
AACGGCC13250.011.44905737
CTCTAAT15050.011.4318941
TATACTG9900.011.398995
TCTATAC8150.011.3496933
TTTAACG13050.011.34099634
Produced by FastQC (version 0.11.5)