FastQCFastQC Report
Fri 29 Apr 2016
ERR1042792.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042792.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391366
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161010.29864416550462347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT154260.28612414738676617No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92250.1711069142773835No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63280.11737285133303879No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG53940.10004885589292214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG13150.017.16349835
TAACGGC13400.016.70522536
GTATTAG22300.015.8452911
ATTAGAG16800.015.5267853
TTTAACG15650.014.89456934
AACGGCC15050.014.87375437
TATTAGA18500.014.82
CGTATTA4550.013.82417515
GTTTAAC17400.013.50287233
CCGTATT4800.013.10416714
CTCTAAT14100.012.8581561
CACATGT18500.012.828
GGTATCA90450.012.7423991
GACACAT18500.012.726
TCGCTAA2052.0705556E-812.63414714
TTAGAGG25400.012.6003934
TGACACA19750.012.55189825
GTGACAC18600.012.53225824
ACCGCCT18250.012.36712312
GGCACCG19150.012.3655359
Produced by FastQC (version 0.11.5)