FastQCFastQC Report
Fri 29 Apr 2016
ERR1042791.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042791.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7131212
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT227380.3188518305163274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT223470.31336889157130654No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129960.18224111132862128No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89630.1256869098829203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG13350.015.24344635
TAACGGC13850.014.69314136
CTAGTAC4400.013.4545453
TATTAGA20950.013.1575182
GTATTAG27400.013.098541
ATTAGAG19000.012.953
TTTAACG17450.012.40401134
TACCCCG8200.011.9573165
GTACTAG3900.011.8589741
TAGCGCG2052.81736E-711.73170729
AACGGCC17350.011.72910637
TACACTG18100.011.6519345
CTCTAAT16850.011.6379811
TAATACT24500.011.62857154
TTAGGAC14600.011.5308223
AATACTG24850.011.4647895
AGGTCGC18250.011.45479535
CTACACT13600.011.4264714
TTAGACT5350.011.4112154
ACCGCCT20800.011.11778812
Produced by FastQC (version 0.11.5)