FastQCFastQC Report
Fri 29 Apr 2016
ERR1042790.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042790.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127180
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61830.5485370570805017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41530.3684415976152877No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.20005677886406784No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC15270.13547082098688762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15020.1332528966092372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG601.3373683E-624.6666685
GTACGTA450.00382576320.55555513
CCGAGGT3900.019.92307732
GCTCCGA4450.019.12359429
GTCGCCC4150.018.7228937
AACGTTC609.236664E-418.526
TCGGAGG4700.017.31914913
GGTATCA23900.017.2615051
AGGTCGC4900.016.98979635
TCCGAGG4850.016.78350431
TAGTACT1005.877586E-616.6500024
GGTATGA1452.9795046E-916.5862061
GGTCGCC4950.016.44444536
GTAAGAT1801.0913936E-1116.4444453
CGAACTA2900.015.94827624
ACACCGT700.002592451915.8571426
TGCTCCG5250.015.85714228
TGTCGTA700.002592451915.85714237
GTAACTC1059.342697E-615.8571423
GTTATAG700.002592451915.8571423
Produced by FastQC (version 0.11.5)