FastQCFastQC Report
Fri 29 Apr 2016
ERR1042789.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042789.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6646283
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT393270.5917141957391824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT352720.5307026498871625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT246870.37144069850772227No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163550.2460773939358285No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG93630.14087573460233338No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC84090.12652184687290624No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC77000.11585423010124607No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG71010.10684167375960367No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG69080.10393779500511789No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC66900.1006577661529008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148300.017.1028331
CTCTAAT26350.015.1650861
AATACTG30750.015.1008135
TAACGGC20050.015.03990136
TTAACGG20250.014.89135835
GTATTAG30700.014.7035851
TCTAATA31550.014.4833592
CTAATAC33800.014.230773
TAATACT33250.014.187974
ATGCTAG26850.013.98696415
GGTAAAC26150.013.93690335
ATACTGG30750.013.7772356
GGGTAAG10650.013.7230041
ATTAGAG23950.013.594993
AACGGCC22650.013.47682137
GATGCTA28800.013.36111114
TACTGGT30750.013.3560977
TAGGACC16750.013.2537314
TATTAGA27650.013.1808322
GTCGTAC2408.185452E-1113.1041671
Produced by FastQC (version 0.11.5)