FastQCFastQC Report
Fri 29 Apr 2016
ERR1042788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5015834
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT164470.3279016012092904No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT157010.3130287007105897No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC96570.1925302950615989No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG78230.15596608659696473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77910.15532810695090787No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG77450.1544110112097011No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC64900.12939024696590834No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGA59090.11780692901718837No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57580.11479646256235754No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG57340.11431797782781487No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC54150.1079581182311855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTT8100.017.5864213
CGAGTTC8750.016.91428614
GCCGAGT8400.016.51785912
GTAAGAT10700.016.079443
GGGTAAG11250.015.951111
ATTAGAG21250.015.8447063
GGTATCA79650.015.8405521
GTCGCCC23750.015.81263237
TTAACGG19250.015.56883135
CGTTCGG1557.226845E-915.51612924
TAACGGC19400.015.25773236
AGGTCGC25100.015.10956235
GTATTAG25150.015.0795231
TATTAGA24250.015.0288672
GCTCCGA26250.015.01142929
AACGGCC20350.014.90909137
TGCCGAG9950.014.68844211
GTGACAC21100.014.6421824
TTTAACG21200.014.39858534
TTTGCCG10100.014.28712759
Produced by FastQC (version 0.11.5)