FastQCFastQC Report
Fri 29 Apr 2016
ERR1042787.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042787.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12192048
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT649370.5326176537362713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT635490.5212331841213224No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT417540.3424691241373065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300040.24609483164764442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA292600.014.7570071
TTAACGG19400.014.68556735
TAACGGC21050.013.53444236
GTATTAG37950.012.9670621
TTTAACG23200.012.83836234
CTCTAAT32350.012.3523961
AACGGCC24350.012.004106537
TAATACT48500.011.9773194
CTAATAC42750.011.8573093
GGGTAAG17250.011.7971011
ATTAGAG29800.011.7332213
TCTAATA40050.011.3632962
CGAACTA25950.011.3352624
CCGAGTT12500.011.24813
TATTAGA39850.011.1882072
TAGGACC21850.011.1762024
TTATGCG18100.011.1408834
GTATCAA389350.011.0662632
AATACTG49650.010.7683795
GGTAAAC32650.010.76569735
Produced by FastQC (version 0.11.5)