FastQCFastQC Report
Fri 29 Apr 2016
ERR1042786.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042786.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15793046
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT913420.5783684793927656No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT911420.5771020992403871No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT596950.3779828159811603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437820.27722327915716827No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159310.1008735110377061No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG28450.013.85061535
GGTATCA445500.013.7659931
TAACGGC30200.013.1092736
GTATTAG52400.012.4627871
CTAATAC51750.012.1903383
GGGTAAG22650.012.1699781
TAATACT56700.011.9417994
CTCTAAT43000.011.9174411
CCGAGTT16850.011.5281913
AATACTG59900.011.5200335
ATTAGAG40150.011.5193023
TCGCTAA4350.011.48275914
AACGGCC35150.011.36842137
TCTATAC18900.011.3544983
TATACTG28550.011.2749565
TTTAACG36200.011.243093534
TTAGGAC32200.011.0310563
TATTAGA50850.011.0235992
TAGGACC31350.010.8580544
TCTAATA52600.010.7975282
Produced by FastQC (version 0.11.5)