FastQCFastQC Report
Fri 29 Apr 2016
ERR1042785.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042785.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15307607
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT756500.49419873400198994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT738920.48271424788995426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT490300.3202982673908469No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT375810.24550538826872154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTAAT42650.013.2731531
TAATACT59700.013.2010054
TTAACGG30850.013.07293335
AATACTG56950.012.9938545
TAACGGC31500.012.74444436
TCTATAC18350.012.5013623
CTAATAC57000.012.4631583
GGGTAAG21650.012.3903011
GTATTAG55700.012.2890481
GGTAAAC42700.011.95784635
TCTAATA52950.011.8791322
CCGAGTT17650.011.63456113
TACTGGT51950.011.609247
ATACTGG55450.011.5103696
GGTATCA442700.011.47108651
AACGGCC35750.011.43636437
ATTAGAG41000.011.41585353
TATACTG21450.011.3846155
TTTAACG37850.011.2906234
TATTAGA51350.011.1324242
Produced by FastQC (version 0.11.5)