FastQCFastQC Report
Fri 29 Apr 2016
ERR1042784.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042784.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12776241
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT695990.5447533433347101No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT668500.5232368425110328No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT463770.36299409192422094No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333260.2608435454528448No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130660.10226795189602325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA311650.015.7011061
CGCGATA1458.929528E-714.03448314
TTAACGG24950.013.64328735
CTCTAAT35650.013.4403921
TAACGGC26000.013.09230736
GTATTAG46800.012.9262831
CTAATAC49750.012.49447253
TCTAATA43000.012.1325582
GGGTAAG17950.011.9554321
ATTAGAG34200.011.9546793
TACCCCG16100.011.9503115
TAATACT51550.011.8428724
TATTAGA42100.011.7767222
GGTAAAC33750.011.7303735
AATACTG51250.011.6234145
GTATCAA428400.011.3616942
TATACTG20750.011.3228915
AACGGCC31350.011.27113237
CCGAGTT13350.011.22471913
TCGCTAA4950.011.21212114
Produced by FastQC (version 0.11.5)