FastQCFastQC Report
Fri 29 Apr 2016
ERR1042783.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042783.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11477222
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT774900.6751633801280483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT716180.6240011738032077No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT393040.34245220664024795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387330.33747713514646666No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153280.13355148136021067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAAG17300.015.7196541
CCGAGTT14250.013.50175413
TTAACGG26150.013.01720935
ATTAGAG33800.012.9718943
GTATTAG43100.012.6624141
TAACGGC27050.012.58410436
CGAGTTC15200.012.53618414
TAATACT48300.012.4865434
CTCTAAT37500.012.2839991
CTAATAC49500.012.2585853
GCCGAGT16000.012.25624912
TCTAATA43400.012.0207382
GACCGTA8700.011.90804526
GGTATCA428250.011.6853471
TACTGGT40150.011.5193037
TTAGAGG50650.011.2497534
AACGGCC30800.011.23214237
ATACTGG46950.011.2300326
TATTAGA40900.011.2176042
GGTAAAC36600.011.17076635
Produced by FastQC (version 0.11.5)