FastQCFastQC Report
Fri 29 Apr 2016
ERR1042782.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042782.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14395026
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT750440.5213189611467184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT739330.5136010174625596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT487220.33846413337495884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT345180.2397911611969301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG26000.013.44807735
GGTATCA386000.013.1417091
TAACGGC26800.013.04664236
GTATTAG50550.012.5895151
TATTAGA42150.011.85053352
CCGAGTT14450.011.77854713
TAATACT52150.011.742094
ATTAGAG35350.011.6181043
CTCTAAT35150.011.4210531
TAGGACC30300.011.295384
GGGGTTA24900.011.2188756
AACGGCC33000.010.87575737
GTATACG4800.010.7916661
ACCGCCT36500.010.69452112
TATACTG18050.010.659285
TTAGGAC30800.010.6314943
TCTAATA43400.010.5714282
TAGGGGT25750.010.5611654
GGGTTAG26750.010.512157
CTAATAC54800.010.431573
Produced by FastQC (version 0.11.5)