FastQCFastQC Report
Fri 29 Apr 2016
ERR1042781.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042781.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9782523
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT431760.44135853296741545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT418760.4280695276668401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT257400.2631223049513914No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203470.20799337757754313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193150.014.5011641
TTAACGG19250.013.93506435
TAACGGC20250.013.24691436
GTATTAG34550.012.9044861
ATTAGAG25200.012.70039753
TAATACT39350.012.6467594
CTCTAAT26850.012.1266291
TATACTG13850.011.6209385
TATTAGA30900.011.4951472
TCTATAC12400.011.4879043
GGGTAAG13000.011.2423091
AACGGCC24150.011.18426537
AATACTG37450.011.164225
TTAGAGG37850.011.143994
GGTAAAC24700.011.08502135
TTTAACG25100.011.05577734
GTGTAAG14950.011.0133771
TCTAATA32650.010.879022
ACCGCCT26700.010.739712
GTATCAA260500.010.7307112
Produced by FastQC (version 0.11.5)