FastQCFastQC Report
Fri 29 Apr 2016
ERR1042780.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042780.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12877936
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT880450.6836887526075608No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT839200.6516572220890056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT561470.43599378036977354No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT463800.36015088132135464No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335560.26056970620136644No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA332160.2579295315646855No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175050.13593016769146857No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG134250.10424807205129767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA301700.019.7079891
CTCTAAT41300.013.6622281
GTATCAA436950.013.5865672
TTAACGG34700.013.38184435
CCGAGTT15900.013.38050313
GGGTAAG21250.013.0588241
TAATACT58550.012.9547414
TAACGGC35750.012.93706336
TCTAATA49150.012.571722
GTATTAG57400.012.4407661
GCCGAGT17450.012.40401112
TAACGCC2255.7243597E-912.3333334
ATTAGAG43850.012.319273
ACCGCCT40650.012.0147612
TATTAGA50650.011.9437312
CTAATAC60750.011.8156393
CGAGTTC17850.011.81512514
AACGGCC40100.011.81047337
TACTGGT49550.011.5741687
GGTAAAC43800.011.44634635
Produced by FastQC (version 0.11.5)