FastQCFastQC Report
Fri 29 Apr 2016
ERR1042779.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042779.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9794572
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2208442.2547590645104245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1394961.4242174134816714No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1061671.0839371031220149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT861610.8796811131716629No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338270.34536475917477555No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT257690.2630947018409789No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234250.23916307930555822No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA219270.22386889391389436No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA134030.13684109933542782No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA133570.13637145145290677No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123580.12617192461293866No Hit
GTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAAAA117350.11981125872575135No Hit
GAGTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA116540.11898427006305126No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT113450.11582946146089897No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA111080.11340975389225788No Hit
GAGTACGGGAAGCAGTGGTATCAACAAAAAAAAAAAAAAAAAA109210.11150053315244404No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102490.10463959017300603No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT21200.021.20518937
CCCACGA26750.020.81682219
TCTCCGA27150.020.6464111
ATCTCCG26900.020.6319710
CACGAGA28400.019.80281821
GCCCACG28700.019.2735218
CGAGCCC29300.019.131415
ACGAGAC29150.018.9125222
GAGACTA29650.018.53119724
GACTAGG29450.018.46859226
AGACTAG30750.017.68780525
CCGAGCC35300.017.5042514
ACTAGGC32250.016.86511627
CGAGACT34050.016.73421523
TATACAC37550.016.6524643
TACACAT38250.016.5411765
TCCGAGC34500.016.51594213
CTAGGCA34650.015.80375328
AGCCCAC36650.015.74897617
TAGGCAT34550.015.74240329
Produced by FastQC (version 0.11.5)