FastQCFastQC Report
Fri 29 Apr 2016
ERR1042778.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042778.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13266098
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT968220.7298453546777659No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT818160.6167299532990033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT495980.3738702970534365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT454640.34270815728935516No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174680.13167398582461853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA392050.013.8260431
TTAACGG21750.013.18390835
TAACGGC22500.012.74444536
TAATACT46800.012.6891034
CCGAGTT12850.012.38132313
GGGTAAG17450.012.2979951
AACGCAG540250.011.9783435
CTCTAAT35950.011.8358831
AATACTG46900.011.7942435
GTATTAG42800.011.4976641
TTAGACT12450.011.4417674
GGTAAAC31450.011.17647135
ATGCTAG33550.010.97317415
CTAATAC49800.010.8473893
ATTAGAC10300.010.7766983
TCTAATA43000.010.7127912
TATACTG21150.010.6713945
TTTAACG29250.010.499145534
GTACTAG7250.010.46206951
TATTAGA39850.010.3989962
Produced by FastQC (version 0.11.5)